Morphological and Molecular Identification of Ticks Infested in Stray Dogs within Nay Pyi Taw Area, Myanmar

Myint Myint Hmoon,Lat Lat Htun,Yu Nandi Thaw,Nyein Chan Soe,May June Thu,Si Thu Aung,Shwe Yee Win,Saw Bawm,Soe Soe Wai,Hla Myet Chel

doi:10.17582/journal.sajls/2018/6.2.41.45

Myint Myint Hmoon, Lat Lat Htun + Show 8 more

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https://doi.org/10.17582/journal.sajls/2018/6.2.41.45

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Abstract

Morphological and Molecular Identification of Ticks Infested in Stray Dogs within Nay Pyi Taw Area, Myanmar

Highlights

Worldwide, there is a growing recognition of the importance of tick-borne pathogens in dogs and humans, whichare transmitted by a number of different ixodid tick species (Nicholson et al, 2010)
Taxonomical identification of ticks was based on the criteria of adult ticks through light microscope (LM) or SEM, the applicability of this method is difficult when the specimens are engorged with blood, physically damaged, in immature stages; i.e. eggs, larvae, or nymphs (Caporale et al, 1995; Guglielmone et al, 2006) and very doubtful at subspecies and/or one group level; R. sanguineus group (Gray et al, 2013; Luiet al., 2013; Nava et al, 2015)
The ticks collected were of the three development stages i.e., larval, nymphal and adult ticks, where adults, nymphs and larvae counted for (217/237) 91.6%, (20/237) 8.4% and (0/237) 0%, respectively.All stages were identified and the species identified were Rhipicephalus sanguineus (237/237).The key characteristics of adult female and male of the brown dog tick, R. sanguineus are as described by Walker et al (2014)

Summary

Introduction

There is a growing recognition of the importance of tick-borne pathogens in dogs and humans, whichare transmitted by a number of different ixodid tick species (Nicholson et al, 2010). Rhipicephalus sanguineus transmits broad rangeof pathogens to dogs such as Babesia vogeli, Babesia gibsoni, Hepatozoon canis, Rickettsia conorii, Rickettsia rickettsii, Ehrlichia canis and Anaplasma platys ( Jongejan and Uilenberg, 2004; Beugnet and Marie, 2009). The morphological identification using light microscope (LM) clarifies mainly the size and color of tick species besides other obvious characJuly-December 2018| Volume 6 | Issue 2 | Page 41 teristics such as mouth parts, the outline of body and scutum (Abdel-Shafy, 2008). Taxonomical identification of ticks was based on the criteria of adult ticks through LM or SEM, the applicability of this method is difficult when the specimens are engorged with blood, physically damaged, in immature stages; i.e. eggs, larvae, or nymphs (Caporale et al, 1995; Guglielmone et al, 2006) and very doubtful at subspecies and/or one group level; R. sanguineus group (Gray et al, 2013; Luiet al., 2013; Nava et al, 2015)

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