Abstract
The soil environment is an important reservoir for a wide variety of entomopathogenic fungi, which can significantly contribute to the control of insect populations, especially on agricultural pests. Comparison of entomopathogens with conventional chemical pesticides, there are advantages in using biological control agents, such as human safety and other non-target organisms; pesticide residues are minimized in food and biodiversity increased in managed ecosystems. The main objective of this study was to detect and identify the entomopathogenic fungi from agriculture soils based on morphological and molecular identification. Total of 115 soil samples were collected in spring (before sowing) from different crop fields in “Unjin” and “Nart” Research and Education Centers of the university, which are located in Bornuur sum, Tuv Province from 2016 to 2018. Isolation of entomopathogenic fungus, Вeauveria bassiana was isolated using Dilution plate method in Peptone Dextrose Agar Yeast. Based on microscopic observation, hyphae branched and formed conidiogene cells, and single cell В. bassiana conidium was round and tend to oval with hyaline color. Colonies on PDAY were white to pale yellow and sometimes red pigments in reverse. Using PCR method with primer specific for identification В. bassiana, molecular analysis confirmed that all six isolates has same size of band which, appeared on agarose gel.
Highlights
Molecular identification of PCR analysis has been done in Genetic Laboratory of Seoul University, South Korea
B. bassianawere isolated from 6 isolates among 570 characterization of B. bassianaisolates are shown on samples fromsoils in fruit, vegetables and wheat Table 1
Based on morphological and molecular characterization of B. bassiana found in different crop fields, the results indicate that agricultural soils are an important reservoir of entomopathogenic fungi
Summary
Synthetic chemical pesticides have ObOevveeenrr ffmeewwainddleeyccaauddseeessd,, ssfyyonnrtthhpeeettisicct ccchhoeenmmtriiocclaa.llHppeeosswttiieccviiddeeers,s hhthaaevvieer bebfeefeeennctsmmoaaiinnnllnyyonuu-ssteeaddrgfefootrropprgeeasstnt iccsomonnstt,rroorlle..siHHduooewwseevvoenerr,,crttohhpeeisirr, epeffeffseetccttrssesooisnntannoocnen--tataanrrdggeecttooonrrcggeaarnniissommvses,,r rrteehsseiiddeuuneevssiroonnmccrreoonpptass,l, pipmeesspttarcreetssiiossfttaannacgceericaaunnlddtucrcaoolnnccienerrpnnutoosvvegerrivttehheeueregnnevvniirrcooynnmmtoeennttthaaell isimmeappraaccchttfooorff aaanggarriilccteuurllnttuuarrtaiavllei,innbppiuoutltsosgggiciivavleelyuubrrgagseeenndccyyforttomo stthhoeef spseeeaasrrtccchhofnfootrroaalnn[1aa]llt.teeIrrnnntaaettriievvseet,s, bbiniioomllooiggciirccoaablllilyaylbbianasseeddctfifcooirrdmmess oaosff pbpeieosstltoccgooinnctatrrloocllo[[n11t]]r..oIIlnnbtteerrtewessettsseniinn1m9m8iic0crraoonbbdiiaa1ll 9iinn9ss0ee,ccuttiisccaiigddeeessanaadss bsbaiioofellootyggiiccaallspccooecnntttsrrooll abbreeettwweedeeinnsc11u99s88se00daannddm11o99r99e00,, udusesaataggieel aanniddn spsaauffbeelttiyycatiaaossnppseeccbttyss Haalrrleeet dadlii.sscc[2uu]ssssaeenddd Lmmaioordrreeet addlee.tt[aa3iill]. Aiinns pspeuuebbnlliitcchaaettiiaoodnnvssabbnyytaHHgeaasllllineettuaaslli..n[[g22m]] aaincnrddobLLiaaiilrrdcdoeentttraaoll..l [a[33g]]e..nAAtsss, stsheeeenn ttihhmeepaaoddrvvtaanncttaaeggeessoifinn uuessniintnoggmmmoiipccarrtoohbboiigaalel cncoosnnttrrhooallsaaggebennetetssn,, ththhiegehliimgmhppteoodrrttaannacscee anooff eneevnnittroommnmooppeaanttthhaoollggyeennfssriehnhadassly bbpeeeeesnnt hchoiiggnhhtrllioigglhhmtteeeddthoaadss [1aa]nn. Eennvviirroonnmmeennttaallllyy ffrriieennddllyy ppeesstt cAcoopnnpttrroolxl mimmeeatththeoolydd [[147]]5..0 species of entomopathogenic AfAuppnppgrriooaxxriiemmkaanttoeelwlyyn 7f7r55o00m s8spp5eegcceiieensseraooffoueenndttootmmhroooppuaagtthhoouggteentnhiicec fcfuulannsggsiieaasrreeofkknnfuoonwwgnni ff[rr4oo]mm. M8855oggsteennoeefrraathffeoouutnanxddottnhhorroomuuiggchhogouruottutthhpees cclolaansstssaeeissn ooff effuunnntoggmii o[[44p]]a..thMMogooesstnt ioocff tthhgeeettnaaexxrooan,noommsiiucccgghrroouuppass cMcooenntttaaariinhniziueemnn,ttoommBeooappuaavtthheoroiggaee,nniiVccertiggceielnnlieeurrmaa,,, Nssouumcchhuraeaaass, MEMneetttoaamrrhhoiipzziihuutmmho,,raBB,eeaaanuudvvNeerreiioaaz,,ygVVieteerrstti,iccaiinlllldiiuufmmew,, oNNtohommeruus.rraaOeenaae,, EoEfnnttohomembooeppshhttt-hhkoonrroaaw,, ananngddeNnNeeerooazzoyygfgiiettenessto,, maannoddpffaeetwhwooogttehhneeirrcss..fuOOnnngeei oiosff tBthheeeabbueevssettr--ikkannoobwwasnnsiggaeennnaee,rraawoohfficeehnnttooismmusooeppdaatthfhooorggeebnnioiiccloffguuinncggaili icissonBBteeroaaluuovveferriiinaasebbcaat sspsseiiasatnnsaa. Wwhhiicchh iissuusseedd ffoorr bbiioollooggiiccaall cGcooennntterroorallloolyff,iinntssheeeccttmppeeassinttss..difference between the most GcGoeemnneemrraaollnllyy,,spttehhceeiemms aaoiifnn eddniitffoffeemrreeonnpccaeethobbgeeettwwnieeceennfutnthhgeeusmmhooassstt ccoommmmoonn ssppeecciieess ooff eennttoommooppaatthhooggeenniicc ffuunngguuss hhaass been characterized based on their shape and size of bcboeeeleonnncychhaaanrraadccttceeorrniizziededdia.bbaHassoeedwd eoovnnertt,hheietiirrissshhsaatipplleepaarnnoddblsseiimzzeeatooicff cdcooulleoontnoyytahanneddreccloaotnniviiddeiilaay.. CIIRnn ittnhhiiossrdssettruuddtoyy,,commnfooillreemccuutllhaaerr araennsaaullylytssiissowfwiittmhhommrpuuhllttoiiplpollegexxicaPPlCCRRidieinnntooifrriddceearrtittooonccoohnnaffsiirrmmbetthehnee rpreeessruufollttrmooeffd.mmoorrpphhoollooggiiccaall iiddeennttiiffiiccaattiioonn hhaass bbeeeenn ppeerrffoorrmmeedd.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
