Morphological and Molecular Evidence for Two New Species within Russula Subgenus Brevipes from China

Jie Song,Bin Chen,Jinyun Zhang,Guang Yang,Junfeng Liang,Qianqian Chen,Shijun Wu,Haijiao Li

doi:10.3390/d14020112

Abstract

Two new Russula species, R. subbrevipes and R. callainomarginis, from China are described based on morphological and molecular characteristics. Russula subbrevipes has thus far only been found in southwestern China at altitudes of higher than 3400 m and is characterized by a yellow ochre pileal surface, glabrous or tomentose stipe, fruity odor, subglobose to ellipsoid basidiospores, isolated or partially connected warts and pleurocystidia with a cap appendage. Russula callainomarginis is characterized by a cream to white pileus, light turquoise lamellae margin, spongy stipe, light turquoise zone on the top of the stipe, pungent odor, globose to ellipsoid basidiospores and dominant isolated warts. The phylogenetic tree of Russula was constructed with multi-gene sequences, including the internal transcribed spacer regions (ITS), the ribosomal large subunit (nrLSU), the small subunit of the mitochondrial rRNA gene (mtSSU) and the second largest subunit of RNA polymerase II (RPB2). The results show that both R. subbrevipes and R. callainomarginis represent new lineages in Russula subg. Brevipes. Description and illustration of the two new species are presented.

Highlights

Russula Pers. was erected by Persoon [1] and typified by R. emetica (Schaeff.) Pers
Bayesian analysis and maximum likelihood (ML) analysis resulted in a similar topology, with an average standard deviation of split frequencies of 0.008966 (BI)
The phylogenetic analyses revealed that the subgenera proposed by Buyck et al (2018) were well-supported with significant Bayesian inference (BI) and ML values: Russula subg

Summary

Introduction

Russula Pers. was erected by Persoon [1] and typified by R. emetica (Schaeff.) Pers. It is an important ectomycorrhizal genus worldwide, comprising more than 2000 species [2,3]. Was erected by Persoon [1] and typified by R. emetica (Schaeff.) Pers. It is an important ectomycorrhizal genus worldwide, comprising more than 2000 species [2,3]. Recent molecular phylogenetic studies based on a worldwide representative sampling have indicated eight subgenera within the genus: Russula subg. Glutinosae Buyck and X.H. Wang; R. subg. Brevipes Buyck and Hofst.; and R. subg. Brevipes Buyck and Hofst was initially recognized as a lineage in the section Plorantinae belonging to R. subg.

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Results

Conclusion