Abstract
Hypodermosis is a distinctive ectoparasitic disease infesting cattle; Hypoderma bovis and Hypoderma lineatum are the most common causes of this myasis. In this study 78 larvae were collected from infected cattle by extraction in the Educational Veterinary Hospital, from Kokjali and Bazwaya flocks and from the skin of slaughtered in Mosul abattoirs for the period from October 2020 to March 2021. Morphological identification by using stereomicroscope depending on patterns of spinulation of the 10th abdominal segment and peritremes structure of L3 classified as H. bovis and H. lineatum. Molecular technique by traditional PCR applied on 16 L3 of the genus Hypoderma revealed that the reaction product was 500 bp by amplification of mt CO1 gene while the results of PCR-RFLP using restriction TaqI enzyme for differentiation between the two species indicated reaction products 300bp for H. bovis and 200bp for H. lineatum respectively. The results of molecular analysis by PCR and PCR-RFLP proved the existence of these two species of Hypoderma in Mosul.
Highlights
Cattle are exposed to different ecto-parasites as ticks [1] and endo-parasites as blood protozoa [2], tissue protozoa such as Neospora caninum and Toxoplasma gondii [3,4] infections
Morphological identification by using stereomicroscope depending on patterns of spinulation of the 10th abdominal segment and peritremes structure of Larvae 3 (L3) classified as H. bovis and H. lineatum
Molecular technique by traditional Polymerase Chain Reaction (PCR) applied on 16 L3 of the genus Hypoderma revealed that the reaction product was 500 bp by amplification of mt CO1 gene while the results of PCRRFLP using restriction TaqI enzyme for differentiation between the two species indicated reaction products 300bp for H. bovis and 200bp for H. lineatum respectively
Summary
Cattle are exposed to different ecto-parasites as ticks [1] and endo-parasites as blood protozoa [2], tissue protozoa such as Neospora caninum and Toxoplasma gondii [3,4] infections. One of the most common ectoparasitic infestation is cattle grub known as cattle hypodermosis resulted from larvae of H. bovis, and H. lineatum [5]. The warble fly infestation (WFI) or Hypodermosis, causes warbles in the subcutaneous of infested animals which considered the distinctive feature for this disease, this myasis is present worldwide and has negative effects on cattle production [5,6]. The discrimination morphologically between the two species H. bovis and H. lineatum based on peritreme structure and on the order of spinulation located on the tenth segment of larvae 3 [8,9]. Molecular analysis by this gene is ideal for identifying 5 species of Hypoderma: H. bovis, H. lineatum, H. diana, H. tarandi, and H. actaeon [10]
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