Abstract
BackgroundBlood parasites of the genus Karyolysus Labbé, 1894 (Apicomplexa: Adeleida: Karyolysidae) represent the protozoan haemogregarines found in various genera of lizards, including Lacerta, Podarcis, Darevskia (Lacertidae) and Mabouia (Scincidae). The vectors of parasites are gamasid mites from the genus Ophionyssus.MethodsA total of 557 individuals of lacertid lizards were captured in four different localities in Europe (Hungary, Poland, Romania and Slovakia) and blood was collected. Samples were examined using both microscopic and molecular methods, and phylogenetic relationships of all isolates of Karyolysus sp. were assessed for the first time. Karyolysus sp. 18S rRNA isolates were evaluated using Bayesian and Maximum Likelihood analyses.ResultsA total of 520 blood smears were examined microscopically and unicellular protozoan parasites were found in 116 samples (22.3% prevalence). The presence of two Karyolysus species, K. latus and K. lacazei was identified. In total, of 210 samples tested by polymerase chain reaction (PCR), the presence of parasites was observed in 64 individuals (prevalence 30.5%). Results of phylogenetic analyses revealed the existence of four haplotypes, all part of the same lineage, with other parasites identified as belonging to the genus Hepatozoon.ConclusionsClassification of these parasites using current taxonomy is complex - they were identified in both mites and ticks that typically are considered to host Karyolysus and Hepatozoon respectively. Furthermore although distortions to the intermediate host erythrocyte nuclei were observed, the defining characteristic of Karyolysus, the haplotypes were nearly identical to those reported from lizards in the Iberian Peninsula, where such distortions were not reported and which were thus identified as Hepatozoon. Based on the phylogenetic analyses, neither vertebrate host, nor geographical patterns of the studied blood parasites could be established.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-014-0555-x) contains supplementary material, which is available to authorized users.
Highlights
Blood parasites of the genus Karyolysus Labbé, 1894 (Apicomplexa: Adeleida: Karyolysidae) represent the protozoan haemogregarines found in various genera of lizards, including Lacerta, Podarcis, Darevskia (Lacertidae) and Mabouia (Scincidae)
Haemogregarina was found in various species of terrapins, while Hepatozoon is the typical apicomplexan parasites found in different species of snakes and lizards [1], each distinguished by very different developmental patterns in their invertebrate hosts in which sporogony occurs
While Hepatozoon is transmitted by a wide spectrum of invertebrates, including hard ticks, transmission of Karyolysus sp. from infected sand lizard (Lacerta agilis) to larvae and nymphs of Ixodes ricinus ticks was not experimentally demonstrated [4], ticks on these lizards are more abundant than mites [9]
Summary
Blood parasites of the genus Karyolysus Labbé, 1894 (Apicomplexa: Adeleida: Karyolysidae) represent the protozoan haemogregarines found in various genera of lizards, including Lacerta, Podarcis, Darevskia (Lacertidae) and Mabouia (Scincidae). Reptiles often serve as hosts for unicellular blood parasites belonging to the suborder Adeleorina, mainly from the genera Haemogregarina, Hepatozoon and Karyolysus. Haemogregarina was found in various species of terrapins, while Hepatozoon is the typical apicomplexan parasites found in different species of snakes and lizards [1], each distinguished by very different developmental patterns in their invertebrate hosts in which sporogony occurs. 1901, belonging to the order Mesostigmata, act as the Haklová-Kočíková et al Parasites & Vectors (2014) 7:555 main vectors These are strictly obligate parasites and can utilize hosts that are taxonomically related (lizards, snakes) [7,8]. Karyolysus represent well defined group different in morphology and in life cycle, it differs from the closely related genera Hepatozoon and Hemolivia in several characteristics of its biology. Intraerytrocytic merogony occurs in Hemolivia, gamonts in the peripheral blood have typical morphology with the presence of a stain-resistant vacuole
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