Morphological and molecular aspects of Ceratomyxa mehlhorni n. sp., a parasite of the golden trevally Gnathanodon speciosus in the Arabian Gulf off the Saudi Arabian coast, with data on its seasonal prevalence.

Abstract

During a survey of the myxosporean fauna of the golden trevally Gnathanodon speciosus from the Arabian Gulf off Saudi Arabia, a species of Ceratomyxa that did not conform to any known species was recorded. The infection was detected as a large number of mature spores free-floating in the bile. Mature spores were hat- or helm-like in the frontal view with two short and unequal valves. The spores measured 8 (7-9) × 12 (10-14). The two polar capsules were spherical, equal in size and measured 3 (2-4) in diameter with three filament turns. Sporoplasm was binucleated and filled the whole extracapsular space. The small subunit ribosomal DNA sequence of this species did not match any available sequences in GenBank. The lowest genetic distance was 0.017, observed with Ceratomyxa moseri infecting Pomacentrus wardi from Australia's Great Barrier Reef. A maximum likelihood phylogenetic tree showed a close association between the new species and a variety of ceratomyxid species, including Ceratomyxa arabica reported from the Arabian Gulf. Given the morphological and molecular differences between this species and other Ceratomyxa spp., we proposed the present form was a new species and the name Ceratomyxa mehlhorni sp. n. for this parasite from the gallbladder of G. speciosus.