Abstract

The purpose of the study was to assess the morphology of red blood cells (RBC) and the association of morphological parameters with lipid peroxidation processes and the content of organic phosphates in RBC when treating packed red blood cells with the ozonized saline solution (with an ozone concentration of 2 mg/l) after different storage periods. Materials and methods. The morphology of human RBC, the concentration of malonic dialdehyde (MDA) in RBC, the catalase activity, the concentration of ATP and 2,3-diphosphoglycerate (2,3-DPG) were studied before and after treatment of RBC with the ozonized saline (with the ozone concentration of 2 mg/l) after 7, 14, 21 and 30 days of storage. Results. The effect of ozone (2 ng/l) in vitro on the packed red blood cells after 7–21 days of storage contributed to the recovery of RBC shape, increased the concentration of ATP and 2,3-DPG, and optimized the lipid peroxidation. Ozone did not demonstrate a pronounced positive effect on these parameters when the packed RBCs were stored for 30 days. Conclusion. The treatment of the packed RBCs with the ozonized saline solution (with the ozone concentration of 2 mg/l) contributed to the recovery of the discocyte count due to optimization of lipid peroxidation processes in cell membranes and enhanced the synthesis of organic phosphates in cells due to the activation of glycolysis and the pentose phosphate pathway. This can be used to improve the morphological and metabolic status of the packed RBCs before their transfusion.

Highlights

  • A loss of 30–50% of the circulating blood volume (CBV) at once is usually not an immediate threat to life, but signifies the onset of severe complications the body can’t cope with

  • We found out that the concentration of ozone of 2 mg/l is most effective in increasing of the ATP and 2,3 DPG concentration in red blood cells after treatment of packed red cells with ozonized saline [11]

  • A significant ability of ozone to influence the ratio of abnormal forms of RBCs and discocytes of the packed red blood cells with the increase of the latter at all storage times was found during the study

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Summary

Introduction

A loss of 30–50% of the circulating blood volume (CBV) at once is usually not an immediate threat to life, but signifies the onset of severe complications the body can’t cope with. Transfusion of packed red cells and recovery of organ blood flow (perfusion) are the most effective measures in the case of a great blood loss due to achievement of a required CBV. It is known that the storage of packed red blood cells causes a decrease in the concentration of ATP and 2,3 DPG in red blood cells and an increase in the content of potassium and lactate in them [1,2,3,4]. The oxygen transport function of red blood cells deteriorates within 24 hours of storage [5]. The deformability of red blood cells decreases by the second week of storage of the packed red cells [6]. It is important to study methods improving the properties of the stored blood

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