Abstract
The current study investigated telocytes (TCs) in the intestinal bulb of Grass carp using light microscopy (LM), Transmission electron microscopy (TEM), scanning electron microscopy, and immunohistochemistry (IHC). By LM, TCs were distinguished by the typical morphological features that had a cell body and telopodes using HE, toluidine blue, methylene blue, Marsland silver stain, Grimelius’s silver nitrate, Giemsa, PAS, combined AB pH2,5/PAS, Crossmon’s and Mallory triple trichrome, Van Gieson stains, Verhoeff’s stain, Sudan black, osmic acid, performic acid with methylene blue and bromophenol blue. TCs were identified under the epithelium as an individual cell or formed a TCs sheath. They detected in the lamina propria, between muscle fibers, around the myenteric plexus and fibrous tissue. TCs acquired immunological features of endocrine cells that exhibited high affinity for silver stain, performic acid with methylene blue, Marsland stain, and immunohistochemical staining using chromogranin A. Sub epithelial TCs were closely related to the endocrine cells. TCs and their secretory activities were recognized using acridine orange. TCs were identified by IHC using CD34, CD117, S100-protein, desmin. TCs formed a3D network that established contact with macrophage, mast cells, dendritic cells, lymphocytes, smooth muscle fibers, fibroblast, Schwann cells and nerve fibers. In conclusion, the localization of TCs in relation to different types of immune cells indicated their potential role in the maintenance of intestinal immunity.
Highlights
The current study investigated telocytes (TCs) in the intestinal bulb of Grass carp using light microscopy (LM), Transmission electron microscopy (TEM), scanning electron microscopy, and immunohistochemistry (IHC)
The current study investigated TCs in the intestinal bulb of the Grass carp
While Tc tend to stain by Verhoeff stain (Fig. 4D) and exhibited a reaction similar to elastic fibers rather than collagen fibers when stained by Combined Weigert counter by Van Gieson stain and Van Gieson (Fig. 4G,H)
Summary
The current study investigated telocytes (TCs) in the intestinal bulb of Grass carp using light microscopy (LM), Transmission electron microscopy (TEM), scanning electron microscopy, and immunohistochemistry (IHC). TCs were identified under the epithelium as an individual cell or formed a TCs sheath They detected in the lamina propria, between muscle fibers, around the myenteric plexus and fibrous tissue. The localization of TCs in relation to different types of immune cells indicated their potential role in the maintenance of intestinal immunity. Telocytes (TCs) are the cell population that forms a network through a labyrinthine system formed by telopodes. They are long and slender prolongations reached up to hundreds of microns. The current study investigates the distribution, morphology, immunohistochemical characterization of TCs in the intestinal bulb of Grass carp
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