Abstract
An entomopathogenic fungus newly named Ophiocordyceps langbianensis was collected from Lang Biang Biosphere Reserve, located in Lam Dong Province, Vietnam. It is characterized as a species of Ophiocordyceps (Ophiocordycipitaceae, Hypocreales) having the unique characteristics of a cylindrical fertile part and several branched apical appendices. Each ascospore develops as two swollen, constricted part-spores. A phylogenetic analysis of multiple genes, including nrLSU, nrSSU, Rpb1, ITS and Tef, supported its systematic position in the genus of Ophiocordyceps; it is related to O. brunneipunctata. Based on morphological and phylogenetic analyses, O. langbianensis was confirmed as a new species from Vietnam.
Highlights
Vietnam is located in a tropical region with terrestrial ecosystems
DL0017 was confirmed as a new species, namely O. langbianensis
During our expedition to validate the diversity of entomopathogenic fungi in Lang Biang Biosphere Reserve, the sample DL0017 was collected
Summary
The isolated genomic DNA was stored in Tris–EDTA buffer at − 20 °C for further studies. The final volume of PCR was done in a total of 15 μL with the thermal program: 1 cycle at 95 °C for 5 min, 40 cycles at 95 °C for 30 s, X °C for 30 s, 72 °C for 2 min, 1 cycle at 72 °C for 5 min (Note: X °C is the annealing temperatures for each target gene shown in Table 1); 5 μL aliquots of amplification product were electrophoresed on a 2.0% agarose gel and visualized in a UV transilluminator. Taxa and nrLSU, nrSSU, rpb[1], ITS and tef sequences collection, DNA proofreading and phylogeny analysis.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
