Morphological and functional changes in random donor platelets stored for seven days in platelet additive solution

Abstract

Aims: Platelets are small, anucleated cytoplasmic fragments that play an essential role in blood clotting and wound healing. Platelets are routinely stored in plasma for five days at 22°C. Aim: Our objective was to assess platelet morphology and function in random donor platelet stored for seven days in platelet additive solution. Methods: The random donor platelet was prepared by PRP method. Platelet concentrates from 50 blood donor were stored in 100% plasma (n=24) and 20:80 (n=24) additive solution. The RDP were placed in incubator and agitator at 22°C. Results: Out of total 50 samples, 48 samples were analyzed and two were discarded due to the bacterial contamination. On comparing the mean values of platelet count and MPV in both the groups with and without platelet additive solution, no significant difference was observed on day seven of storage period. A significant difference in PDW was observed on day seven (p < 0.001) in plasma. The mean values of LDH and pH showed no significant difference on day seven in both the groups with and without platelet additive solution. A significant difference was observed in the levels of glucose on day seven (p < 0.001) in plasma. In platelet aggregation, a significant decrease was seen in plasma (p < 0.001) on day seven at 22°C. Conclusions: The platelet morphology and function was better maintained in additive solution on day seven as compared to platelets stored without additive solution.