Morphological and electrophysiological properties of serotonin neurons with NMDA modulation in the mesencephalic locomotor region of neonatal ePet-EYFP mice.

Abstract

The mesencephalic locomotor region (MLR) is an essential area for initiation of locomotion. Its functional roles and circuits underlying locomotion have been studied intensively in many species. Studies suggest that cuneiform nucleus and pedunculopontine nucleus (PPN) are two core regions in the MLR for locomotion. However, it remains unclear about cellular components and morphological and intrinsic membrane properties of the neurons in these regions, especially the serotonergic neurons. Using neonatal ePet-EYFP transgenic mice and immunofluorescent technique, we demonstrated existence of 5-HT neurons in the MLR and discovered that 5-HT neurons distributed mainly in the caudal PPN. 5-HT neurons were heterogeneous in MLR and had three types of firing pattern (single spike, phasic and tonic) and two subtypes of morphology (pyramidal and stellate). We measured parameters of 5-HT neurons (n = 35) including resting membrane potential (- 69.2 ± 4.2mV), input resistance (1410.1 ± 616.9MΩ), membrane capacitance (36.4 ± 14.9 pF), time constant (49.7 ± 19.4ms), voltage threshold (- 32.1 ± 7.4mV), rheobase (21.3 ± 12.4 pA), action potential amplitude (58.9 ± 12.8mV) and half-width (4.7 ± 1.1ms), afterhyperpolarization amplitude (23.6 ± 10.4mV) and half-decay (331.6 ± 157.7ms). 5-HT neurons were intrinsically different from adjacent non-5-HT neurons and less excitable than them. Hyperpolarization-activated inward currents and persistent inward currents were recorded in 5-HT neurons. NMDA increased excitability of 5-HT neurons, especially the tonic-firing neurons, accompanied with depolarization of membrane potential, hyperpolarization of voltage threshold, reduction of afterhyperpolarization half-decay, and left-shift of frequency-current relationship. This study provided insight into the distribution and properties of 5-HT neurons in the MLR and interaction between serotonergic and glutamatergic modulations.