Abstract
Abstract The cell content of the hemolymph from the two species of stick insects Bacillus rossius and Carausius morosus was characterized in morphology and cytoskeletal distribution by flow cytometric analysis, phase‐contrast microscopy, and scanning electron microscopy. In in vitro cultured hemocytes, the cytoplasmic distribution of F‐actin microfilaments was revealed with Rhodamine‐labelled phalloidin, and that of microtubules by indirect im‐munofluorescence with a monoclonal anti α‐tubulin. The results show that both stick insects possess peculiar hemocyte sub‐populations, as detected by flow cytometry, composed of plasmatocytic and granulocytic cells. Carausius morosus has a hemocyte subtype, the spherulocyte, that appears to be lacking in Bacillus rossius. The results of cytoskeletal analysis show that the plasmatocytes of both species, smeared on glass coverslips and compared with cultured mammalian epithelial control cells, possess a regular cytoskeletal network, with F‐actin microfilaments organiz...
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