Morphological and biological assessment of films based on hyaluronic acid doped with cerium oxide and aluminum oxide for wound healing applications

Abstract

Developing wound bandages with advanced properties to be qualified in improving the period of healing to avoid the harmful inflammation and the danger of the side effects that may be caused by the long period. Hyaluronic acid (HA) was fabricated and doped with several oxides; Alumina oxide (Al2O3) and Cerium oxide (CeO2) via film casting method. Furthermore, XRD, FTIR, SEM, TGA, water contact angle, and cell viability were used to characterize the fabrication process of the scaffolds. Rough and porous surface were a mutual morphological properties for all the fabricated scaffolds which may lead to good adhesion and easy transfer of therapeutic fluids and moisture movement through the wound. TGA shows a great thermal stability for CeO2/Al2O3/GO@HA according to the three stages of degradation that the scaffold went through. These stages started from temperature under 200 °C to the final degradation stage that occurred at 599.2 with mass loss about 67 % of total components. Furthermore, the assessment of cell viability was employed to evaluate the biological compatibility of the scaffold with normal lung cells for a period of three days in vitro. The concentration of the substance was first set at 2500 μg/ml, and the Ic50 value was subsequently discovered to be 625 μg/ml. The decrease in drug concentration is associated with an increase in cell growth that estimated by 95.3 % with 4.4 μg/ml of concentration.