Morphological and Biochemical Evaluation of the Testes of Hyperglycemic Guinea Pigs Treated with Cyperus Esculentus Extract

Abstract

In this study, we aimed to evaluate the effects of Cyperus esculentus extract on Testicular Morphology and Biochemistry post streptozotocin-treatment in Male Guinea Pigs. Twenty guinea pigs were divided into four groups containing five pigs each. Group A served as the negative control was treated with 5ml/kg body weight of normal saline orally, Group B were treated with a single dose of 65mg/kg body weight of streptozotocin (STZ) intraperitoneally, Group C were treated with a single dose of 65mg/kg body weight of STZ and daily treatment with 150mg/kg of CEE orally and Group D were treated with a single dose of 65mg/kg body weight of STZ and daily treatment with 150mg/kg of CEE orally for 20days. Morphological scores and biochemical evaluation were done. Results showed a significant decrease in testicular weight, number of spematogonia, Leydig cells and Sertoli cells, levels of SOD and CAT as well as a significant increase in the level of MDA after the administration of STZ alone when compared to the control. A significant decrease in the levels of testosterone and FSH as well as a decrease in the level of LH was observed in group B when compared to the control. However, the groups that were co-treated with the extract had a better parameters and histological profiles when compared to the positive control group. CEE has a dose-dependent ameliorative effect on STZ-induced testicular hormonal, oxidative and histomorphometric degeneration.