Morphological and biochemical changes in mucous cells of the murine sublingual salivary gland during the carbamylcholine-induced secretory cycle

Abstract

Changes in these cells have been evaluated over 6 h following cholinergic stimulation. Carbamylcholine administration resulted in the release of almost 50 per cent of secretory material within 15 min, which caused a reduction of 33 per cent in cell size. After 2 h the cells were depleted of secretory material. However, in the second hour the release of secretory material was accompanied by an enlargement of the nucleus, Golgi complexes and rough endoplasmic reticulum (RER), which suggests an elevation of biosynthetic activity. The enlargement of the RER was not the result of an increase in RNA, i.e. in the number of ribosomes, but of dilatation of its cisternal spaces. Before release took place, there was a continuous coalescence of secretory granules. After this extensive fusion, which is probably the result of an altered physiological state of the granule membrane and subsequent water uptake caused by cholinergic stimulation, the viscous mucins could be squeezed out, water transport is likely to assist in this ejection. Refilling of the mucous cells was almost complete within 6 h after stimulation.