Abstract
Optimization of plants long-term conservation under in vitro conditions and identification of their structural and functional features during preservation is the basis for the creation of a genebank of cultured and wild plant species in vitro. The aim of our work was to study morphological and anatomical features of plants in lavender cultivar ‘Sineva’ after two years of in vitro storage at low positive temperatures. The plant material was evaluated after 24 months of conservation. A significant decrease in the linear parameters of leaves in the preserved plants was noted. Leaf shape altered from narrowly linear to oblong. With prolonged storage, the thickness of the leaf blade slightly increased. The integumentary tissues were thinner, compared with the plants cultured under standard in vitro conditions, the cuticle was not clear. The number of stomata decreased. The number of small vascular bundles decreased. The obtained data demonstrate viability of in vitro preserved explants, despite significant differences in structure, preservation of chlorophyll-containing tissues, a decrease in transpiration, and an increase of trichomes number. The stability of the vegetative organs functioning was revealed.
Highlights
The main task in biological diversity conservation is the comprehensive studies and preservation of genetic resources by replenishing and maintaining collections of living plants, as well as long-term conservation of explants that ensure their viability and stability [1, 2]
Scientific experience indicates that plants, in various culture conditions, can both preserve morphology and anatomy characteristics of a particular genotype, and acquire new features [3,4,5]
Our previous studies revealed the main parameters that affect the reduction of the growth kinetics and the preservation of the physiological stability in lavender plants under 24-month conservation: culture medium 1⁄4 MS supplemented with 0.2 mg L-1 CCC and 60.0 g L-1 sucrose, temperature - 6°С
Summary
The main task in biological diversity conservation is the comprehensive studies and preservation of genetic resources by replenishing and maintaining collections of living plants, as well as long-term conservation of explants that ensure their viability and stability [1, 2]. Optimization of the conditions for in vitro plant long-term conservation and identification of structural and functional features under their storage is the basis for the conservation of valuable genotypes and the creation of the in vitro plant genebank [6, 8, 9]. Constant replenishment of the collection requires new approaches to in vitro preservation of the valuable genotypes, including the analysis of the adaptive capacity in the explants of particular plant species and cultivars under conditions of prolonged conservation
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