Abstract
BackgroundTo evaluate the morphology and immunohistochemical expression of MMP-9, TIMP-3, COL-I, TNF-alfa and COX-2 in the dentinopulp complex of restorations with self adhesive composite (Vertise Flow/Kerr) compared with conventional resin composite with self-etching adhesive (Filtek Z250/3M ESPE and Clearfil SE Bond/Kuraray-Noritake). Material and MethodsA randomized, controlled, double-blind, split-mouth, two-arm clinical trial was conducted. Twenty-seven volunteers with third molars indicated for extraction received two deep class I restorations, one with each material. The morphology and immunohistochemical expression were measured at 15 days after the restorative procesures. The data were submitted to the Fisher`s Exct test (α=0.05). ResultsThrough morphological analysis, a slight disorganization of the odontoblastic layer was visualized, along with a slight inflammatory infiltrate in the VERT and CSEB groups (p< 0.05). In immunohistochemical analysis, TNF-alpha, MMP-9, TIMP-3 and COX-2 demonstrated more cases of positive pulp labeling. ConclusionsSelf-adhesive resin composite and conventional resin composite with a self-etching bonding agent promoted slight alterations in the dentin-pulp complex, indicating a repair ability and reversibility of the inflammatory process against aggressions of the adhesive restorative procedure. Key words:Immunohistochemistry, inflammation, randomized clinical trial, self-adhering composite, dentistry.
Highlights
Adhesive restorations may be performed with different hybridization strategies that use complete smear layer removal by an etch-and-rinse adhesive system, or smear layer modification by a self-etch and self-adhesive system [1]
One of advances in dentistry is the introduction of self-adhering flowable composite resins (SACs) which are a product of combining an all-in-one bonding system and flowable composite resin
MMPs have the capacity of degrading components of the extracellular matrix (ECM), such as collagen I (COL-I), compromising the quality of the restoration and reducing the biocompatibility of these materials [6]
Summary
Adhesive restorations may be performed with different hybridization strategies that use complete smear layer removal by an etch-and-rinse adhesive system, or smear layer modification by a self-etch and self-adhesive system [1]. The composition of restorative materials may trigger an inflammatory response [5] Cytokines such as cyclooxygenase-2 (COX-2) are released as well as growth factors (tumor necrosis factor alpha - TNF-alfa) and proteolytic enzymes (such as matrix metalloproteinases – MMPs). This study is aimed to evaluate the morphology and immunohistochemical expression of MMP-9, TIMP-3, COL-I, TNF-alfa and COX-2 in the dentinopulp complex of restorations with SAC (VERT group) (Vertise Flow/Kerr) compared with conventional resin composite with self-etching adhesive (CSEB group) (Filtek Z250/3M ESPE and Clearfil SE Bond/Kuraray-Noritake). To evaluate the morphology and immunohistochemical expression of MMP-9, TIMP-3, COL-I, TNF-alfa and COX-2 in the dentinopulp complex of restorations with self adhesive composite (Vertise Flow/Kerr) compared with conventional resin composite with self-etching adhesive (Filtek Z250/3M ESPE and Clearfil SE Bond/Kuraray-Noritake). Conclusions: Self-adhesive resin composite and conventional resin composite with a self-etching bonding agent promoted slight alterations in the dentin-pulp complex, indicating a repair ability and reversibility of the inflammatory process against aggressions of the adhesive restorative procedure
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
