Morphological analyses of sperms causing severe male infertility with light microscopy, motile sperm organellar morphology examination and electron microscopy tecnics.

Abstract

To evaluate abnormal sperm morphology by three different methods including light microscope, real-time motile sperm organelle morphology assessment at 8050x high magnification and electron microscope and to determine degree of affect on morphologic defects level. Beside the light and electron microscopic techniques, the motile sperm organelle examination can offer information about sperm quality in a simple and quick way and by the IMSI developed through the use of sperm selected by this technique can contribute to the success of in vitro fertilization centers. We believe that sperm selection at high magnifications can increase IVF pregnancy rates in patients with sperm morphology that causes severe male infertility.