MORPHOLOGIC INVESTIGATION OF SUBCHRONIC FORMALDEHYDE INHALATION ON RAT BRAIN TISSUE

Abstract

Ponte Academic JournalNov 2021, Volume 77, Issue 11 MORPHOLOGIC INVESTIGATION OF SUBCHRONIC FORMALDEHYDE INHALATION ON RAT BRAIN TISSUEAuthor(s): Rabia Tasdemir ,Tuncay Çolak, Belgin Bamaç, Süreyya Ceylan, Selenay Furat RençberJ. Ponte - Nov 2021 - Volume 77 - Issue 11 doi: 10.21506/j.ponte.2021.11.4 Abstract:Background/Objectives: The purpose of our study was to investigate the effect of formaldehyde (FA) on different parts of rat brain tissue. For this purpose, we determined BDNF and S100 expression levels by immunohistochemical methods and examined cell death via TUNEL method in rat brains exposed to subchronic FA doses. \nMethods: 24-Wistar albino rats were randomly divided into three groups: control group (CG, n=8), low dose FA group (LDG, n=8) and high dose FA group (HDG, n=8). LDG and HDG were exposed approximately 1 ppm and 10 ppm FA, respectively. Implementations lasted for 12 weeks. Inhalation period was 5 days a week and 4 hours a day. Finally, rats were sacrificed and brain tissues were gently removed. Brain tissue sections were stained with Haematoxylin-Eosin (H&E) for histopathologic examination whereas TUNEL method, BDNF and S100 antibodies were performed for immunohistochemical examination under light microscopy. \nResults: There was a significant decrease in body weights of rats belonging to HDG compared to the CG and LDG (p?0,05). In cortex, significant difference was detected between CG and LDG in terms of BDNF immunreactivity, whereas in thalamus and hippocampus, significant difference were seen between CG and both FA groups (p?0,05). Similarly, in cortex, there was a statistically significant difference between all groups for S100 immunreactivity. In thalamus and hippocampus, significant difference was only seen in LDG and HDG (p?0,05). In the TUNEL analysis, a statistically significant increase was observed in the apoptotic index values of both FA groups compared to the control group.\nConclusions: According to our findings, in FA groups, BDNF expression decreased while S100 expression increased. Apoptotic cell index results also supported increased S100 immunreactivity in FA groups. We suggested that FA exposure damaged to the different parts of brain tissue, caused neuronal-glial cell death and altered protein expressions. Download full text:Check if you have access through your login credentials or your institution Username Password