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Abstract
Comparative ultrastructural studies of changes in the morphologies of invertebrate eggs that occur after fertilization may shed light on the evolution of the fertilization process and the incorporation of mechanisms that may serve to insure monospermy as well as to provide protective envelopes for the developing embryo. This report describes changes that occur in the egg of Mercierella enigmatica as noted 35 minutes after fertilization.Eggs or sperm were shed spontaneously when the worms were pulled from their tubes and placed in individual watch glasses containing sea water. The unfertilized eggs were fixed for 1 hour in 2.5% glutaraldehyde in 0.1M cacody- late buffer at pH 7.5. The eggs were rinsed in sea water and post fixed for 1 hour in 1% osmium tetroxide buffered with 0.1M phosphate buffer at pH 7.5. Other eggs were fertilized by the addition of sea water containing sperm and fixed as above after 35 minutes. Eggs were embedded in Epon and compared by TEM. Embedments of the unfertilized eggs and fertilized eggs were fractured and coated with vacuum evaporated gold for SEM.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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