Abstract
Fresh and dry weights and leaf size of Poa pratensis were reduced when treated with 6-azauracil (AzU), (2-chloroethyl)phosphonic acid (CEPA), or (2-chloroethyl)trimethylammonium chloride (CCC). AzU and CEPA inhibited epidermal cell division without inhibiting cell elongation, while CCC inhibited mainly cell elongation and cell division to a small extent. The ratio of blade length to sheath length and the blade length/width ratio were reduced, but leaf emergence and tillering were increased by AzU and CEPA. CCC affected only the latter three features. Like GA3, CEPA induced stem formation, but internodes were shorter. GA3 was ineffective in preventing leaf-growth inhibition by AzU, which inhibited Ga3-induced cell elongation. The inhibitory effect of CEPA on leaf growth was apparently reversed by GA3, but this was due solely to increased cell elongation, the reduction in cell number being unaffected. Ga3 reversed the effect of CCC on leaf length, as well as on cell size and number. Simultaneous application of the inhibitors produced a complex interaction in reducing leaf length and number and size of epidermis cells. It is postulated that AzU, CEPA, and CCC have different modes of action because they have specific effects on plant growth and different effects on GA3-induced cell elongation.
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