Abstract
Aim. To obtain planting material of Miscanthus × giganteus in a large amount in order to study the peculiari- ties of morphogenesis of isolated meristem culture. Methods. The explants were sterilized using 70 % etha- nol and 0.1 % mercury bichloride, after that, the methods of plant cultivation and reproduction in vitro and statistical processing of the obtained data was performed. Results. The study presents the results of obtaining the regenerate plants from dormant buds of 3-year-old rhizomes of Miscanthus × giganteus. The Murashige and Skoog medium supplemented with 6-benzylaminopurine (BAP) (0.75 mg/L) and kinetin (1.2 mg/L) was found to be the best for regeneration of microsprouts, providing the maximum value of the regeneration index (95 %) and contributing to the development of the main sprouts and tillering. The obtained sprouts were rooted in Murashige and Skoog medium supplemented with half-dose of macro- and micronutrients without growth regulators. In doing so, rooting index made up 95.0–97.0 %. The best substrate for the adaptation of miscan- thus plants in vivo was found to be a mixture of peat, sand, and perlite in a ratio of 2 : 1 : 1, respectively. In this substrate, plant establishment reached 91 %. Conclusions. The peculiarities of morphogenesis in Miscan- thus × giganteus isolated meristem were studied. At the same time, the index of sprouts regeneration varied from 90.0 to 100.0 %. In addition, the development of the main sprout and multiple tillering occurred in 85.0– 100.0 % explant. The highest survivability of micro-plants (91–95 %) was provided in the peat-sand-perlite substrate in a ratio of 2 : 1 : 1.
Highlights
One of the top priority tasks of all the civilized countries is ensuring the development of bioenergetics, preserving natural resources and searching for new sources of alternative energy
To obtain planting material of Miscanthus × giganteus in a large amount in order to study the peculiarities of morphogenesis of isolated meristem culture
Our studies demonstrated that the highest percentage of sterile dormant buds (70 %) was obtained by keeping them consistently in 70 % C2H5OH (1.5 min) with further transfer into 0.1 % solution of HgCl
Summary
One of the top priority tasks of all the civilized countries is ensuring the development of bioenergetics, preserving natural resources and searching for new sources of alternative energy. By-products of plant origin (straw, corn stalks, etc.) and dedicated energy plants may be used as energy commodity [2, 3] One of these plants is a tree-like grass – miscanthus. The experience of Europe demonstrates that miscanthus has high biological performance of biomass in a wide geographical range of moderate climatic regions, including marginal lands [7, 8]. It is not grown much in Ukraine yet, the interest to this energy crop is increasing consistently, as it can yield up to 20 t/ha of dry matter in favorable weather conditions
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