Abstract
Tooth pulps dissociated intact with EDTA were isografted for up to 40 days, and examined by light and electron microscopy for hard tissue morphodifferentiation. Grafts formed tubular dentine and osteodentine. Tubular dentine, penetrated regularly by elongated odontoblast processes, resembled normal dentine and was formed when the original odontoblasts continued normal matrix secretion. Osteodentine was formed by spindle-shaped cells with large round nuclei which presumably were transformed pulp cells, and incorporated the same elements as found in cells of non-tubular dentine. Occasionally, odontoblasts were contiguous with both the regular dentine and the osteodentine. Thus in EDTA-dissociated pulps transplanted to the spleen, the original odontoblasts produce tubular dentine and other pulp cells differentiate to form osteodentine.
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