Abstract
Explants from Sideritis angustifolia Lag. were evaluated for morphogenesis when cultured on MS medium supplemented with various concentrations of spermidine, dicyclohexylamine, NAA, IAA and BA. Spermidine by itself did not induce morphogenesis. However, this polyamine reversed the toxic effect on callus and root proliferation caused by 27.0 μM NAA or 28.5 μM IAA. Dicyclohexylamine, an inhibitor of spermidine synthesis, reduced both callus growth and rhizogenesis on hypocotyls cultured in the presence of 10.8 μM NAA or 11.4 μM IAA. The effects of spermidine on BA-induced growth was similar to those previously stated with auxins, especially in relation to the neutralization of toxicity elicited by 10.8 or 27.0 μM BA. Hypocotyl-derived calli obtained in the presence of 0.01 mM spermidine and 27.0 μM NAA gave rise to pro-embryonal masses and free embryoids when transfered to liquid medium without growth regulators. These pro-embryonal masses proliferated quickly when placed on hormone-free solidified medium and subsequently regenerated both shoots and complete plantlets. Once the developed shoots and plantlets were excised, the remaining calli maintained this regenerative capability in successive subcultures.
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