Morphogenesis in vitro Flower Pedicel of Lilium longiflorum with NAA and BAP

Abstract

Lilies are one of the cut flowers most favored by consumers. Each lily plant can produce several flower stem, and each flower consists of many flower buds. Flower bud stems have the potential as explants in vitro culture. Propagation in vitro is a very promising technique for plant propagation. It has the advantage of being able to produce a lot of seedlings in a short time. Pedicel explants are reported to be the best explants after the petal to produce shoots and callus, but it is not yet known which pedicel age is best for explants. The aim of the study was to obtain information about the effect of NAA and BAP concentrations on Vacin & Went (VW) media and age of explant of flower stalks (pedicels) on morphogenesis of lilies in vitro. Method of this study is in vitro technique on VW media with complete randomized trial design. The results showed that explants of flower stems (pedicels) that were used in vitro culture were pedicel buds compared to pedicels with blooming flowers and withered flowers. Younger plants still have meristem tissue. The meristem tissue produces the auxin hormone which helps in cell division for faster bud growth The best concentrations produced in this study were 1 ppm NAA and BAP in percentage of growth, time of callus initiation, number of shoots, number of browning and browning time and 0,5 ppm NAA + 1 ppm BAP for time of shoot initiation. The result of 1 ppm NAA and BAP showed the best response because the composition of the media by using a combination of growth regulators from a group of auxins and cytokinin in a balanced amount can initiate cell enlargement and induction callus.
 Keywords: lily, pedicel, age, explant, stem