Abstract
There has been a widespread interest in cell, tissue and organ culture techniques for clonal propagation (1), haploid production and breeding (2), and mutagenesis (3). In spite of the large amount of work being done in this field there are many limitations of the method. One of the most important limitations has been a lack of detailed knowledge regarding the precise conditions of culture and control of differentiation in cell and organ cultures of important crop plants. Different species of Brassica have attracted worldwide attention because of their economic importance as sources of oil and vegetables (4–10). The present report pertains to the control of differentiation in tissue cultures of two oil yielding species B. campestris var. DYS-1 (Rape), B. juncea var. Prakash (mustard) and two vegetable crop plants B. oleracea var. botrytis (cauliflower) and B. oleracea var. capitata (cabbage).
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