Morphogenesis and fibronectin in sexual differentiation of rat embryonic gonads.

Abstract

The possible role of fibronectin in the organization of the sex-specific gonadal components was studied by immunocytochemistry combined with electron and light microscopy in rat fetuses at the ages of 12-15 days. Fibronectin was evenly distributed in both sexes under the basal lamina of the surface epithelium. Other basal laminae were not seen using light or electron microscopy inside the gonadal ridges at the age of 12 days. As the first sign of sexual differentiation, fibronectin-negative gonadal cords appeared in 13-day-old fetuses. In the males the cords were bigger than those in the females. The cords were clearly separated from the interstitium in 15-day-old fetuses of both sexes. A continuous layer of fibronectin had formed between the testicular surface epithelium and the elongated cords indicating the formation of a tunica albuginea. In females the surface epithelium-cord connection was maintained at all stages. Connections of the gonadal cords to mesonephric tubuli were seen in the rete region of both sexes. The electron optical basal lamina around the gonadal cords became continuous by the age of 15 days. The present results suggest that fibronectin is intimately involved in the sexual differentiation of the gonads, but not under the regulation of H-Y antigen or other testis-organizing factor.