MORPHOFUNCTIONAL PARAMETERS OF CATTLE ERYTHROCYTES UNDER STRESS AND ITS CORRECTION BY LOW-INTENSITY LASER RADIATION

Abstract

The study investigated the content of malondialdehyde (MDA), the electrophoretic mobility of erythrocytes (EPME), the spectrum of erythrocyte membrane proteins and the morphology of cattle erythrocytes in in vitro experiments under stress and exposure to low-intensity laser radiation (LLLT). Clinical and experimental studies carried out in the last decade indicate the possibility of modulating the organism adaptive reactions when exposed to such physical factors as low-intensity laser radiation. The work showed that the effect of LLLT on the blood of stressed animals caused the restoration of the studied parameters to the level of physiological norms, while in animals that underwent technological stress, EPME was reduced by 31%, MDA concentration was increased by 65%. The effect of LLLT on the blood of unstressed animals did not lead to a change in EPME and MDA concentration. The study of the protein spectrum of erythrocyte membrane of animals subjected to technological stress revealed that the content of spectrin decreased by 16%, glycophorin C increased by 35%, the morphology of erythrocytes after stress was characterized by an increase in the number of echinocytes, stomatocytes and degeneratively altered erythrocytes. Under the LLLT action on the cow erythrocytes after stress there was a restoration of the morphology of cells and the content of proteins of erythrocyte membranes to the control level.