Abstract
Aim. To fi nd out the contents and placement of CD4+, CD8+, CD45RA+, CD20+-lymphocyte subpopulations and morphocytochemical architecture of the harderian gland in clinically healthy chickens and for vaccination against infectious bronchitis. Methods. Anatomical, histological, immunohistochemical, optical, morphometric and variational-statistical. Results. The obtained morphometric study results showed that the area of secretory lobules and lymphoid formations was signifi cantly larger in the harderian gland of vaccinated 40-, 90- and 110-day-old chickens. Moreover, the organ size correlates signifi cantly with the development of lymphoid formations, since the area of the secretory lobules in the gland of the experimental group chickens does not differ from that of the control group. Immunohistochemical studies have shown that the number of lymphocytes with CD20+ markers was characterized by a reliable (P < 0.001) increase in B-lymphocytes in the harderian gland of the vaccinated chickens of all ages. Lymphocytes with CD8+ markers were not detected in the gland of vaccinated 8- and 20-day-old chickens. According to cytomorphometric studies, their number in vaccinated 40-, 90- and 110-day-old chickens was signifi cantly (P < 0.001) higher compared to intact birds – 2.11, 2.38 and 2.96 times, respectively. Conclusions. The data on the composition of lymphocytes with CD4+, CD8+, CD45RA+, CD20+ markers in the harderian gland of chickens of different age, on their changes in case of immunization against infectious bronchitis were obtained, and the role of certain clusters at different stages of the immunity formation was determined. The information on the structure, patterns of growth and development of the harderian gland of chickens, which demonstrates the incompleteness of its morphogenesis at the early stages of the postnatal ontogenesis period that should be taken into account during the compilation of vaccine prophylaxis programs, has been supplemented.
Highlights
ObjectivesThe aim of the work was to investigate the morphofunctional condition of the harderian gland in chickens at vaccination for prophylaxis purposes
The organometric investigations demonstrated that the absolute weight (AW) of the harderian gland in 8-day-old chickens of the control group was 0.017 ± ± 0.0007 g with further increase up till 40 days: in 20-day-old chickens – up to 0.068 ± 0.002 g (Р < < 0.001); in 40-day-old chickens – up to 0.086 ± ± 0.002 g
Starting with the age of 90 days, there was a decrease in the AW of harderian gland (HG) down to 0.073 ± 0.004 g (Р < 0.01), and at the age of 110 days this index, compared to the previous group, decreased 1.52 times and amounted to 0.048 ± 0.002 g (Р < 0.001)
Summary
The aim of the work was to investigate the morphofunctional condition of the harderian gland in chickens at vaccination for prophylaxis purposes
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