Morphofunctional characteristics of adipose-derived multipotent mesenchymal stromal cells from CBA/Ca mice of different ages in cell culture in vitro

Abstract

In modern regenerative medicine, the use of adipose-derived multipotent mesenchymal stromal cells (ADSCs) is an important strategy for the treatment of many diseases, including age-dependent ones. However, the question of a direct link between the aging of ADSCs and the aging of the body as a whole remains open. The purpose of the study was to determine the morphofunctional properties of multipotent mesenchymal stromal cells of adipose tissue in young and old mice when cultured in vitro. Materials and methods. Subcutaneous adipose tissue was isolated from young (2 months old) and old (14 months old) female CBA/Ca mice, fermented, and the stromal vascular fraction was obtained. ADSCs were isolated after cell culturing for 2nd passage. Cells were phenotyped by flow cytometry and morphological characteristics, proliferative potential, ability to directed multilinear differentiation in osteogenic, adipogenic directions were evaluated in vitro. Results. ADSCs obtained from old animals after the second passage showed a decrease in the proliferative, clonogenic potentials of ADSCs in vitro compared to the cells from young animals. In addition, the osteogenic potential of ADSCs in vitro decreased and the potential for adipogenic differentiation increased. Conclusions. The results of the study indicate an age-dependent effect on the functional activity of ADSCs, which is manifested by a disorder of their potential for proliferation, colony formation and directed differentiation.