Morphofunctional changes in the reproductive organs of females against the background of the use of a phytopreparation (experimental study)

Abstract

The paper presents the results of the assessment of morphological changes in the uterus and ovaries of laboratory animals against the background of the introduction of therapeutic doses of a multifunctional phytopreparation, which includes alcohol extracts from plants: white hellebore (Veratrum album), dioecious nettle (Urtica dioica), yarrow (Millefolii herba) and has rumination, uterotonic, hemostatic and anti-inflammatory effects. Therapeutic doses for cows were tested, which for rats amounted to 0.2 ml per head. The phytopreparation was adminitered subcutaneously three times, every other day. The control group rats were subcutaneously injected with a saline solution of sodium chloride (NaCI 0.9%) according to the same scheme. The methods of digital microscopy were used in the study of micropreparations. Morphometric analysis of the histoarchitectonics of the uterus revealed a statistically significant increase in the diameter of the cross-section of the uterus of female rats of the experimental group by 25.8% (p≤0.05) compared with the control group due to an increase in the epithelial by 24.5% (p≤0.05) and the endometrial lamina of the uterine mucosa by 32% (p≤0.05), which indicates on increasing the secretory activity of the epithelial cover of the uterus. In the experimental group of rats treated with phytopreparation, a significant increase of 46% (p<0.05) in the number of primordial follicles was noted. There was also a tendency to increase the number of primary – by 36.3%, secondary – by 41.7%, tertiary – by 75% of follicles and yellow bodies – by 22.2% in rats of the experimental group compared with the control indicators. Quantitative indicators of the follicular composition of the ovaries of rats of the experimental group and an increase in the follicle aging coefficient by 60% (p≤0.05) indicate stimulation of the ovarian reserve against the background of the use of phytopreparation.