Morpho-physiological and transcriptome analysis provide insights into the effects of zinc application on nitrogen accumulation and metabolism in wheat (Triticum aestivum L.)

Abstract

Nitrogen (N) is the essential nutrient for wheat growth and development, its accumulation and metabolism controlled by many other elements. Zinc (Zn) is one of the important elements which tends to have effects on plant N homeostasis. Here in our study, 0 μM and 5 μM Zn was applied to the wheat seedlings culturing in 5 mM (+N) and 0.5 mM (-N) N treatments, respectively. The results showed that the shoot and root length growth performance, total N, NO3−, and amino acid concentrations, glutamine synthetase (GS) activity of wheat were facilitated by 5 μM Zn application under + N and –N conditions. Quantitative real-time PCR (qRT-PCR) analysis indicated that several NO3− transporters genes (TaNRT2.1, TaNPF7.1 and TaNPF7.2) and the genes encoding GS (TaGS1 and TaGS2) were induced by 5 μM Zn. In addition, transcriptional changes in wheat shoots and roots with Zn application were tested by RNA-seq techniques. A total of 147/551 induced and 36/2162 reduced differentially expression genes (DEGs) was detected in wheat shoots/roots, respectively. GO and KEGG enrichment analyses showed that 5 μM Zn mainly affected the glutathione (GSH) metabolism, phenylpropanoid biosynthesis and amino acid metabolism, involving in N homeostasis. Furthermore, the relative expression of genes related to phenylalanine, cysteine and methionine metabolism was induced by 5 μM Zn to promote the amino acid accumulation. Overall, these results highlight the facilitating of N accumulation by low level Zn, and provide an insight into the effects of Zn on N metabolism in wheat.