Abstract
This study aimed at identification and characterisation of the wilting, leaf spot, stem canker and fruit-blight diseases of okra. Pathogen infected plant samples were sterilised with 2% sodium hychlorite solution for 20 mins and then cultured at 28 °C for 15 days followed by microscopic examination. Combined microscopic, morphological and cultural characteristics analyses identified the four okra fungal isolates (OFI) such as OFI-1, OFI-2, OFI-3 and OFI-4 as Fusarium oxysporum, Curvularia lunata, Fusarium chlamydosporum and Choanephora cucurbitarum, respectively. The isolates OFI-1, OFI-2, OFI-4 showed highest radial growth rates at 25 °C, pH 5 and 20% lactose containing media while it was sucrose for OFI-3. BLASTN search using the sequence of ITS regions revealed that OFI-1, OFI-2, OFI-3 and OFI-4 isolates had 98, 100, 97 and 100% homology with F. oxysporum, C. lunata, F. chlamydosporum and C. cucurbitarum, respectively. Pathogenicity tests were also confirmed by reproducing the disease symptoms in okra plants.
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