Morpho-Histology and Morphometry of Chicken Testes and Seminiferous Tubules among Yellow-Feathered Broilers of Different Ages.

Abstract

Simple SummaryTestes are important male reproductive organs that in chickens have been greatly investigated, from pre-hatch to after sexual maturity. The present study investigated the changes in components that occur during growth, and evaluated morphometry of the seminiferous tubules (ST), as well as gonadosomatic index (GSI) in Gallus domesticus at different age stages. The left and right testes were harvested from 70 chickens, then fixed in alcoholic acetate formalin (AAF) fixative solution, and hematoxylin- and eosin-stained tissues were used for microscopic observations. The results revealed that the left testis (LT) and the right testis (RT) exhibited fuzzy ST features, with apoptotic resorption of many tubules observed in both testes of 1-wk-old chicks only. ST formation was completed at 1 month, with an increase of all morphometric parameters in both testes until sexual maturity (3-mo-old): the age at which we recorded the greatest GSI. This study provides details on ST apoptotic resorption, which is a process not yet reported in existing publications, as well as ST morphometry and GSI, from a juvenile stage of growth towards sexual maturity. This can serve as reference material and also as a data update to better understand the morpho-histological changes that occur in chicken testes during growth.Unlike in many mammals, poultry testes are found in the abdominal cavity where they develop and perform spermatogenesis at high body temperature. Scarce reports among current publications detail the growth of testes and ST morphometry among juvenile chicks. Therefore, this study aims to investigate changes in components occurring in Gallus domesticus testes, by assessing the GSI and morphologically and histologically evaluating the testes and ST morphometry from 1-wk- to 4-mo-old. Right and left testes were collected from 70 healthy chickens divided into seven age-related groups (n = 10) and then immersed into the alcoholic acetate formalin (AAF) fixative solution. Hematoxylin- and eosin-stained tissues were used for microscopic observations. The findings revealed that both testes exhibited smooth features from 1-wk-old to 1-mo-old, and thereafter showed a consistent increase in vascularization until 4-mo-old. Histologically, both testes exhibited unclear ST, with ST apoptotic resorption observed in the 1-wk-old chicks. Until 1-mo-old, ST formed and few spermatogonia differentiated into primary spermatocytes, with all spermatogenic cells observed at 3-mo-old, i.e., sexual maturity. These findings suggest that both testes develop in analogy, and their sizes including increases in length and diameter are related to the spermatogenic activity in the ST. Subsequently, ST resorption by apoptosis is assumed to participate in the physiological mechanism regulating germ cells (GC). Finally, the GSI tended to increase with growth.