Morpho-Histological Analysis of Direct Shoot Organogenesis Induced in Flower Buds Cultures of &amp;lt;i&amp;gt;Allium altissimum&amp;lt;/i&amp;gt;

Tatyana V Poluboyarova,Tatyana I Novikova,Elena V Andronova,Galina Yu Vinogradova

doi:10.4236/ajps.2014.513216

Abstract

The present work gives a detailed study of in vitro shoot organogenesis of the ornamental onion A. altissimum Regel from the buds of the middle layer of the inflorescences. The course of morphogenesis was examined by light and scanning electron microscopy. Histological observation revealed that during 3 - 5 days of culture on the BDS medium supplemented with 2.0 mg·L-1 of BA and 2.0 mg·L-1 of NAA the epidermal cells of the stamen filament in the area of its fusion with the tepal became competent and dedifferentiated. Originally the organogenesis involved several initial epidermal cells. The formation of meristematic centers was observed from day 3 to day 14. The apical shoot meristems and leaf primordia in a roller shape formed from day 14 to day 28 of culture on the same media. The further development of vegetative shoots and formation of the bulblets were observed when the explants were stimulated by triapenthenol (2.0 mg·L-1).

Highlights

De novo shoot organogenesis in tissue culture is the key process for both clonal micropropagation and fundamental studies of developmental biology [1] [2]
We have developed the protocol of micropropagation from the flower organs of another anzur species A. altissimum [14]
Initiation and differentiation of the generative shoots are genetically controlled by the plant, which allows turning the apical meristems of the shoots into meristems of the flower organs [18] [19]

Summary

Introduction

De novo shoot organogenesis in tissue culture is the key process for both clonal micropropagation and fundamental studies of developmental biology [1] [2]. (2014) Morpho-Histological Analysis of Direct Shoot Organogenesis Induced in Flower Buds Cultures of Allium altissimum. The two initial phases are of major interest in terms of developmental biology [5]. A. altissimum is used as an ornamental plant in landscape design and flower arrangements. Mass propagation of this geophyte is limited by the characteristics of its life cycle: the plants develop slowly from the seeds and reach the generative phase in 6 - 8 years [6]. The vegetative propagation is absent or poorly expressed: during vegetation the mother bulb can form one, rarely two daughter bulblets [7]

Methods

Results

Conclusion