Abstract

A new high performance liquid chromatography-chemiluminescence analysis system for morphine is described. This system has an on-column limit of detection of 20 pg for morphine, allowing morphine determination down to 1 ng/mL, with a 20 μL injection size, without any concentration during sample preparation. Simultaneous determination of monoacetylmorphine to 15 ng/mL (300 pg on-column) is also achieved. The internal standard for both compounds is nalorphine and total analysis time is under 5 minutes.

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