Moringa Oleifera mitigates the Fluoxetine-induced damage to the histological architecture of the seminiferous tubules in adult male rats

Abstract

Objective: To observe the ameliorative changes of Moringa oleifera on the gross and histological architecture of fluoxetine-treated adult rat testis. Methodology: This experimental study was conducted in Department of Anatomy, Army Medical College, Rawalpindi in collaboration with the National Institute of Health (NIH), Islamabad and Pathology Lab Pak Emirates Military Hospital Rawalpindi, Pakistan, from October 22 to April 2023. Thirty male Sprague Dawley rats were randomly divided into three groups (n=10). Rats were kept at the animal house of NIH Islamabad. Group A (control), Groups B and C (experimental) were given doses dissolved in distilled water through oral gavage once daily. The experimental period was 08 weeks. Group B was administered fluoxetine in a dose of 10 mg/kg body weight. Group C rats were treated with two chemicals, same dose of fluoxetine as in group B and concomitantly received 50 mg/30 grams body weight of Moringa Oleifera. After dissection, testes were fixed and staining was done with Hematoxylin & Eosin for histological study. Analysis of data was done on SPSS version 22. A p-value ? 0.05 was considered statistically significant. Results: The present study concluded that amelioration caused by Moringa Oleifera was statistically significant (p-value ?0.05) when compared to the other groups. Conclusion: Moringa Oleifera has ameliorative effects on the testicular toxicity induced by Fluoxetine in male rats by showing improvement in the diameter of the seminiferous tubules.