Moringa oleifera leaf extract ameliorates cisplatin evoked neurotoxicity in male wistar rats

Abstract

The study was intended to explore the potential role of Moringa oleifera leaf extract (MOLE) on cisplatin (CIS) induced neuronal damage in experimental male rats. In the current work, animals were divided into four groups, six animals in each group. Group-1 (Normal) administered with Distilled water 1ml p.o. Group-2 (CIS) was received cisplatin 5mg/kg, ip. single dose. Group-3 (CIS + MOLE 200mg/kg) was orally administrated MOLE 200mg/kg, dissolved in water for 14 days with a cisplatin 5 mg/kg, ip. single dose on 10 day and Group-4 (CIS + MOLE 400mg/kg) was orally administrated MOLE 400mg/kg, dissolved in water for 14 days with a cisplatin 5mg/kg, ip. single dose on 10 day. After 14 days of MOLE dosing, the rats were sacrificed after anesthetizing with ketamine. Brain of individual rat was excised and processed for evaluation of oxidative injury markers like malonyl dialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase and histopathological study. MOLE treated experimental groups explored a potential (P<0.001) protection against CIS induced neurotoxicity by elevating the GSH, SOD, catalase and suppressing MDA in the brain as well as amelioration of histopathological changes induced by CIS at different doses. Thus, investigational finding revealed that MOLE possess potential benefits against neurotoxicity induced by anti-cancer drug cisplatin by regulating oxidative stress markers and ameliorating neuronal death and alteration of microanatomy of rat brain.