Abstract
The influence of morin hydrate on changes of proliferative, metastatic, and adhesive potential of human ovarian cancer cells concerning the influence of decitabine, and decitabine with trichostatin A, and in comparison to untreated cells, were analyzed. The effect of morin hydrate, decitabine, and trichostatin A were examined in A2780 and SKOV-3 ovarian cancer cell lines using MTS assay, clonogenic assay, adhesion to endothelial HMEC-1 cells, transwell migration assay and cell cycle analysis. The expression level of epithelial to mesenchymal transition (EMT) markers was quantified using PCR Array in relation to the level of global methylation determined with Methylated DNA Quantification Kit. We observed statistically significant inhibition of adhesive and migratory potential of both cell lines and the accumulation of G0/G1 phase A2780 cells after treatment with morin hydrate. Our studies confirmed the influence of morin hydrate on down-regulation of genes considered as up-regulated during EMT, and up-regulation of some genes considered as down-regulated during EMT in A2780 and SKOV-3 cells. Phenotypic changes were associated with molecular changes in cells, eg. decrease of the expression level of genes associated with adhesion, and an increase of genes down-regulated during EMT, after morin hydrate treatment in comparison to untreated control cells in both cell lines, were observed.
Highlights
Epigenetic basics of epithelial to mesenchymal transition (EMT) in cancerEpigenetic aberrations, including DNA methylation, histone modifications, and microRNA dysregulation play an important role in the development and progression of ovarian cancer
The effect of morin hydrate, as well as decitabine and trichostatin A on ovarian cancer cells, were analyzed using MTS assay after treatment for 24 h at various concentrations
In SKOV-3 cells IC50 was 45.64 μM ± 1.9 μM for decitabine, 29 nM ± 0.78 nM for trichostatin A, and 388.57 μM ± 3.2 μM for morin hydrate
Summary
Epigenetic aberrations, including DNA methylation, histone modifications, and microRNA dysregulation play an important role in the development and progression of ovarian cancer. We hypothesized that morin exerts the antiproliferative, anti-metastatic, and anti-adhesive influence on ovarian cancer cell lines in reference to the changes of expression level of genes involved in epithelial to mesenchymal transition, and the methylation level in cells, similar to decitabine, and decitabine in combination with trichostatin A as the example of chemical epigenetic modulators. The ΔCq value for each sample was determined by calculating the difference between the Cq (quantitation cycle) value of the genes of interest and the Cq value of the reference housekeeping genes (e.g., RPLP0—Ribosomal protein, large, P0 for A2780 cells, HPRT1—Hypoxanthine phosphoribosyltransferase 1 for SKOV-3 cells, and B2M—Beta-2-microglobulin for both) used for normalization, according to the manufacturer’s protocol. A p values less than 0.05 were considered as statistically significant
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
