Abstract
Knowledge on the anatomical structure of Hoya coronaria Blume can help to understand the various function of the plant to support the success of Hoya cultivation outside its natural habitat. This study aims to examine the effect of differences in light intensity on the anatomical characteristics of yellow-flowered H. coronaria to evaluate the adaptation of H. coronaria outside its natural habitat. The experiment used a one-factor factorial completely randomized design with two light intensity treatments (highest daily light intensity 15 Klux) and no shade with the highest daily light intensity average 59 Klux). The preparation of the leaf paradermal incision was made using the whole preparation method while the transverse incision was made using the paraffin method. Based on the analysis of the anatomical structure of the leaves, H. coronaria is a plant with C3 photosynthesis type. Leaf anatomy is composed of a thick cuticle layer, a single layer of the epidermis, mesophyll tissue which is differentiated into palisade and sponges, has a wide intercellular air cavity, and there is a transport network with undeveloped vascular sheath cells. H. coronaria growing under higher light intensity had thicker upper and lower cuticle, lower epidermis, mesophyll layer, and spongy layer compared to lower light intensity. The chlorophyll content was not significantly different between those grown at low and high light intensities. Based on these results, it can be concluded that H. coronaria adapted to high light intensity (without shade) or locations exposed to direct sunlight.
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