Abstract
presence or absence of anaerobic glycolysis (i.e. glucose or galactose in the culture medium). Glucose allows ATP to be produced by both aerobic and anaerobic glycolysis, whereas galactose only allows cells to produce ATP by mitochondrial oxidative phophorylations. Data was analysed by the Student’s t-test or the Mann–Whitney U-test as appropriate. Results: In the presence of Glucose/10% FCS, the maximal cell count was (mean±SEM, ×106 cellules) 2.79±0.31 vs. 0.33±0.04 in immature & adult ASMC respectively (p< 0.05). DNA synthesis (thymidine incorporation) in immature AMSC (fold increase vs. ITS control) was 6.55±1.02 vs. 2.70±0.43 in adult ASMC (p< 0.05). A similar trend was observed with PDGF-AA (2.83±0.62 vs. 1.47±0.12, p < 0.05). This was not observed in the presence of the other agents. C/EBPa & TRPC1 expression were similar in immature & adult ASMC. DNA synthesis in Glucose-free (Galactose)/10% FCS medium were much greater in immature vs. adult ASMC (4.02±0.80 vs. 0.34±0.07 fold increase, p < 0.05). Conclusion: Enhanced proliferation of immature ASMC in the presence of 10% FCS or PDGF-AA appears to be related to a mitochondrial-dependent mechanism, similar to what has been reported in adult asthmatic ASMC. This may contribute to early airway remodelling.
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