Abstract
This work is devoted to the simulation of human skin autofluorescence in different spectral ranges. Analytical review was performed for selecting the main endogenous fluorophores with the greatest contribution to the skin fluorescence: tryptophan, tyrosine, collagen, melanin, elastin, lipofuscin, protoporphyrin IX, NADH, FAD. It was necessary to set parameters for autofluorescence modeling, such as the absorption/emission spectra of fluorophores, molar concentration, molar extinction coefficient, and quantum yield. The six-layer skin model was designed in the TracePro software and autofluorescence was simulated when excited at different wavelengths in the middle UV (270-300 nm), near UV (330-360 nm) and visible (400-450 nm) spectral ranges. The simulation results were compared with the experimental results of other authors. The principal distinctive factor of this work is the simulation of the human skin autofluorescence excited in different spectral ranges.
Highlights
Autofluorescence (AF) of the skin is the fluorescent radiation of natural fluorophores inside the tissue excited by absorption radiation with a specific wavelength
This paper demonstrates the dependences of the shape and intensity of AF spectra by excitation radiation at different wavelengths in three spectral ranges
The skin model in this range contained five fluorophores that were excited in the UV range: tyrosine, tryptophan, elastin, NADH, Protoporphyrin IX
Summary
Autofluorescence (AF) of the skin is the fluorescent radiation of natural fluorophores inside the tissue excited by absorption radiation with a specific wavelength. It is possible to reproduce the spectral-optical properties of a biological object in a model experiment. In this case, the model can simulate the spatial structure, composition, and localization of the fluorophores in a real tissue. Many fluorophores have similar or overlapping absorption and emission spectra, with the result that the tissue fluorescence has a complex spectral composition. In this regard, there is uncertainty with the excitation wavelength and with the emission wavelength for each specific fluorophore [3]. The aim of this work is AF simulation of human skin model for interpreting experimental spectra (estimating the fluorophores contributions) and assessment of various tissue states (inflammation, neoplasm, etc.)
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