Abstract
Only one place available: Monovalent streptavidin is prepared in a one-step process based on a trisbiotinylated oligonucleotide that blocks three of streptavidin's four biotin-binding sites. The complex is highly sensitive to single-base differences: perfectly matched oligonucleotides trigger dissociation of the biotin–streptavidin interaction at higher rates than strands with single-base mismatches.
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