Monolayers, bilayers and micelles of zwitterionic lipids as model systems for the study of specific anion effects

Abstract

This work is a review of the effect of different monovalent anions (belonging to the Hofmeister series) on a single model amphiphile, phosphatidylcholine molecules in contact with water, in three different geometries, Langmuir monolayers, bilayers and micelles, which differ by area per molecule and radius of curvature. The zwitterionic lipids DPPC (1,2-dipalmitoyl-phosphatidylcholine) and DPC (Dodecylphosphocholine) have been used to form the three geometries. The salt solutions used were NaCl, NaBr, NaNO 3, NaI, NaBF 4, NaClO 4 and NaSCN. Various interfacial and bulk experimental techniques were used in order to study the effect of the anions on the amphiphiles in the three geometries. The interfacial techniques used were surface pressure–area isotherms, Brewster angle microscopy (BAM), Grazing incidence X-ray diffraction (GIXD), infrared reflection absorption spectroscopy (IRRAS). The bulk sensitive techniques used were small-angle X-ray scattering (SAXS), osmotic stress (OS) and light scattering. The experimental results obtained from the three model systems indicate that the interfaces become charged through lipid-ion association. The results were fitted with appropriate theoretical models to provide “binding” or “partitioning” constants of anions in the headgroup region of the lipid molecules. The theoretical analysis strongly suggests that the anions do not associate with the lipid molecules through a local chemical reaction, but rather they partition inside the disordered lipid interfaces.