Abstract
The spreading monolayers of ceramides alone and in mixtures with monooleoyl glycerol were studied at the air/water interface in the 15-30°C temperature range. The bidimensional phases were determined from the spreading isotherms of the pure components, whereas the interfacial miscibility of the compounds being studied was deduced from the spreading isotherms of the mixtures. The specific resistance and capacitance of black lipid membranes containing mono-oleoyl glycerol and monooleoyl glycerol/ceramides in squalene were studied. Langmuir-Blodgett monolayers and bilayers obtained by the transfer of spreading monolayers at the air/water interface onto chromated glass slides, were prepared. Vesicles of dioleoylphosphatidylcholine and of mixtures of dioleoylphosphatidylcholine/ceramides were also prepared. The results obtained for the different types of aggregates were compared in order to find the relations that connect the characteristics of the various systems. Results showed that the possibility of obtaining flat and curved bilayers of pure components and of mixtures was closely connected to the phase of the corresponding monolayers at the air/water interface.
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