Abstract
Monolayer behavior of the dye 7-nitro-2-1,3-benzoxadiazol-4-yl (NBD)-labeled analogues of phospholipids 1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine (DPPE) and 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) were studied by a variety of methods. Attachment of the NBD chromophore onto either the phospholipid headgroup or the aliphatic chain significantly changes the parent phospholipid monolayer properties. In contrast to that of the condensed-type DPPE monolayer, the isotherm of the DPPE-NBD derivative with NBD in the headgroup exhibits a liquid-expanded/liquid-condensed phase transition plateau while the isotherm of the acyl-chain-labeled NBD(C12)-PC shows liquid-expanded behavior lacking the plateau observed in the isotherm of DPPC. Surface potential and spectroscopic data revealed that the NBD group on both DPPE-NBD and NBD(C12)-PC embeds into the phospholipid headgroup region of the monolayer, thus suggesting a looping of the NBD-labeled chain of NBD(C12)-PC toward water. Polarized fl...
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