Abstract
Injury to sugarbeet, Beta vulgaris L., from sulfonylurea herbicide residues from preceding cropping years has kindled interest in developing resistant cultivars. This study was conducted to obtain chlorsulfuron (2‐chloro‐N‐[[(4‐methoxy‐6‐methyl‐1,3,5‐triazin‐2‐yl)amino]carbonyl]benzenesulfonamide) resistance from cell cultures and to determine its inheritance and magnitude. Utilizing annual diploid sugarbeet clone REL‐1, dispersed suspension cultures were initiated from callus induced on leaf disk cultured on a modified Murashige and Skoog (MS) agar medium + 1.0 mg L−1 N6‐benzyladenine (BA) and placed in the liquid form of the same medium. Unmutagenized cell clusters were plated on solid medium containing 2.8 nM chlorsulfuron in MS + 1.0 mg L−1 BA. A single colony arose, from which shoots were regenerated. Shoots were resistant to 28 nM chlorsulfuron, a concentration that killed similar shoots of REL‐1. Resistance (designated Sur) was inherited as a monogenic dominant. In vitro shoot resistance to chlorsulfuron was 300 to 1000‐fold greater than in REL‐1. Resistance was also expressed in leaf disk expansion in vitro with MS + 1.0 mg L−1 BA.
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