Monoethanolamine Treatment of Fish Wastes and Salmon Guts to Increase It Palmitoylethanolamide and Anandamide Contents

Abstract

This study was carried out to determine the palmitoylethanolamide (PEA) and arachidonoylethanolamide (AEA) or anandamide contents in selected fish wastes, treating the fish wastes with highest PEA and AEA with different concentration of monoethanolamine (MEA) solution, incubation temperature and time, as well as the ratio of MEA solution to fish waste to further increase its PEA and AEA contents.
 Based on the results of the preliminary experiment, a fractional factorial design experiments was done with 4 factors including MEA concentration, incubation time, incubation temperature and dosing ratio (MEA solution:salmon guts). The results showed that the MEA content ranged from 2.25 to 8.06 mg/g sample, the PEA content ranged from 17.4 to 300.2 µg/g sample while the AEA content ranged from 1.3 to 19.0 µg/g sample all on a wet weight basis of all the FD treated samples. The FD treated sample with the highest MEA, PEA and AEA using an MEA solution concentration of 250mM from pure MEA chemical, incubation time of 0.5 hour, incubation temperature of 6oC and a dose ratio of 6 mL MEA solution:100 g salmon guts. The MEA, PEA and AEA contents of the different samples were analysed using the Yates algorithm to determine which of the four factors were more important. The results showed that MEA, PEA and AEA contents were significantly affected by the concentration of MEA solution used in dosing the salmon guts, followed by the incubation time and then a slight effect of dosing ratio while the incubation temperature has no significant effect.