Abstract
The aim of present study was to investigate the relationship between phenotypic features of monocytes and intensity of “respiratory burst” in the patients with renal cell carcinoma (RCC). A total of 73 patients with RCC (Т3N0М0, clear cell type) participated in the study. Phenotyping of blood monocytes was performed by flow cytometry. The level of “respiratory burst” in monocytes was determined using spontaneous and zymosan-induced luminol- and lucigenin-dependent chemiluminescence. Suffficient changes in phenotypic structure and intensity of “respiratory burst” were identified in peripheral blood monocytes of the patients. Alterations of monocytic subpopulations in RCC were characterized by increased numbers of cells with the CD14 low CD16 + (“non-classical”) phenotype. The imbalance in expression of activation markers was found among monocyte populations from cancer patients; we have revealed a reduced number of monocytes expressing HLA-DR-antigen, and increased number of CD64-positive cells. Meanwhile, intensity of “respiratory burst” in the total monocyte population proved to be reduced in RCC patients. In this case, the characteristic features of the “respiratory burst” intensity distribution among monocytes were as follows: in RCC, a reduced “respiratory burst” activity was found in monocytes with CD14 + CD16 - phenotype, being, however, increased in the monocytes with CD14 + CD16 + and CD14 low CD16 + phenotypes. Such redistribution may be due to increasing role of the given monocyte subsets in immunopathogenesis of renal cell carcinoma.
Highlights
Следует отметить, что моноциты периферической крови, рассматривавшиеся долгое время в качестве единой группы клеток, на основании функциональной активности и экспрессии некоторых поверхностных антигенов подразделяются на несколько различных популяций [2, 12, 37]
The imbalance in expression of activation markers was found among monocyte populations from cancer patients; we have revealed a reduced number of monocytes expressing HLA-DR-antigen, and increased number of CD64-positive cells
Ziegler-Heitbrock L., Ancuta P., Crowe S., Dalod M., Grau V., Hart D.N., Leenen P.J., Liu Y.J., MacPherson G., Randolph G.J., Scherberich J., Schmitz J., Shortman K., Sozzani S., Strobl H., Zembala M., Austyn J.M., Lutz M.B. Nomenclature of monocytes and dendritic cells in blood
Summary
ФЕНОТИПИЧЕСКИЙ СОСТАВ И ХЕМИЛЮМИНЕСЦЕНТНАЯ АКТИВНОСТЬ МОНОЦИТОВ У БОЛЬНЫХ ПОЧЕЧНОКЛЕТОЧНЫМ РАКОМ. Савченко А.А.1, Борисов А.Г.1, Модестов А.А.2, Мошев А.В.1, Кудрявцев И.В.3, 4, Тоначева О.Г.2, Кощеев В.Н.1. У больных ПКР установлены изменения в фенотипическом составе и интенсивности «респираторного взрыва» моноцитов периферической крови. Кощеев, «Фенотипический состав и хемилюминесцентная активность моноцитов у больных почечно-клеточным раком» // Медицинская иммунология, 2015. For citation: A.A. Savchenko, A.G. Borisov, A.A. Modestov, A.V. Moshev, I.V. Kudryavtsev, O.G. Tonacheva, V.N. Koshcheev, “Subpopulations and chemiluminescent activity of monocytes in patients with renal cell carcinoma”, Medical Immunology (Russia)/ Meditsinskaya Immunologiya, 2015, Vol 17, no. Savchenko A.A.a, Borisov A.G.a, Modestov A.A.b, Moshev A.V.a, Kudryavtsev I.V.c, d, Tonacheva O.G.b, Koshcheev V.N.a a Research Institute for Medical Problems of the North, Krasnoyarsk, Russian Federation b Krasnoyarsk A.I. Kryzhanovsky Regional Clinical Oncology Center, Krasnoyarsk, Russian Federation c Research Institute of Experimental Medicine, St. Petersburg, Russian Federation d Far Eastern Federal University, Vladivostok, Russian Federation
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