Abstract
AbstractThe culture medium of a human acute monocytic leukemia cell line (THP‐1) was able to induce normal human monocytes to spread. No such ability was found either in the culture medium of a promyelocytic leukemia cell line (HL‐60) nor in that of a diploid human fibroblast cell line (Flow 7000). Gel filtration of the culture medium of THP‐1 cells on a size exclusion column (TSKgel G3000SW) revealed that the most obvious monocyte spreading activity was found in the fraction eluted at the position with a molecular weight of about 70,000. This fraction was also able to increase production of angiotensin converting enzyme and Fc receptor sites for IgG on normal human monocytes.
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